Effect of grain processing and silage on microbial protein synthesis and nutrient digestibility in beef cattle fed...

Effects of the extent of grain processing and the percentage of silage in barley-based feedlot diets on microbial protein synthesis and nutrient digestibility were evaluated using four steers (initial BW of 442 +/- 15 kg) with ruminal and duodenal cannulas. The experiment was a 4 x 4 Latin square with four periods of 21 d each. Dietary treatments were arranged as a 2 x 2 factorial with two levels of barley silage (20 and 5% DM basis) and two degrees of barley grain processing (coarsely and flatly steamrolled to a processing index [PI] of 86 and 61%, respectively). The PI was quantified as the volume weight of the barley grain after processing, expressed as a percentage of the volume weight prior to processing. Digest a flow (Yb) and microbial (15N) markers were continuously infused into the rumen for a period of 13 d. Ruminal, duodenal, and fecal samples were collected at various times over the last 6 d of marker infusion. Diurnal ruminal pH was measured for 48 h. Intake of DM averaged 1.8% of BW, and was not different among the dietary treatments (P > 0.10). Ruminal starch digestibility was higher (P < 0.05) for the more extensively processed grain and tended (P < 0.10) to be highest when the more extensively processed grain was combined with 5% barley silage. In contrast, ruminal fiber digestibility for the 5% silage diets was reduced (P < 0.05) when the grain was more extensively processed. There was, however, no effect of grain processing on ruminal OM digestibility (P > 0.10), and hence, no inhibitory effect on microbial N flow to the intestine (P > 0.10). There was also no effect of the level of silage on microbial N flow (P > 0.10), but there was a tendency for improved efficiency of microbial protein synthesis for the 20% silage diets (P = 0.072). Ruminal escape of nonmicrobial N (P = 0.003) was greater, and thus, protein flow to the intestine was greater for the 5% silage diets. Diurnal ruminal pH was lower (P < 0.05) for 11 of the 24 hourly time points in steers fed the 5% silage diets than those fed the 20% silage diets. In conclusion, barley grain rolled to a PI of 86 to 61% and combined with 20 and 5% barley silage had little effect on microbial protein supply. Microbial protein supply was not inhibited when the barley grain was extensively processed (PI of 61%) and the silage was limited to only 5% of the diet DM, but feed intake of steers in this study was lower than would be expected in the feedlot.