Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Oviduct cells express the cyclic AMP-adenosine pathway.

Barbara Cometti1, Raghvendra K Dubey, Bruno Imthurn

  • 1Departments of Medicine and Pharmacology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15261, USA.

Biology of Reproduction
|May 2, 2003
PubMed
Summary

The extracellular cAMP-adenosine pathway is present in oviduct cells. This pathway converts cyclic adenosine monophosphate (cAMP) to adenosine, potentially influencing reproductive functions like fertilization and embryo development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Purinergic activity of circulating extracellular vesicles associates with disease progression in melanoma.

Oncoimmunology·2026
Same author

Rationale for the Use of 8-Aminoguanine for the Management of Cystitis.

International journal of urology : official journal of the Japanese Urological Association·2026
Same author

8-Aminoguanine protects against paclitaxel-induced neural degeneration and mechanical allodynia.

JCI insight·2026
Same author

Effects and Mechanism of 2'-AMP on Renal Vascular Resistance: Role of P2X1 Receptors.

Journal of the American Society of Nephrology : JASN·2026
Same author

CD39 polymorphism enables lung thrombosis in sickle cell disease.

Nature communications·2026
Same author

Purine Nucleoside Phosphorylase Inhibition Rebalances Purine Metabolism and Attenuates Organ Damage in Sickle Cell Mice.

Journal of cellular and molecular medicine·2025

Area of Science:

  • Reproductive Biology
  • Cellular Physiology
  • Biochemistry

Background:

  • The extracellular cAMP-adenosine pathway involves the conversion of cyclic adenosine monophosphate (cAMP) to adenosine.
  • This pathway's presence and function in oviduct cells are not well-established.
  • Adenosine plays roles in various physiological processes, including reproduction.

Purpose of the Study:

  • To determine if the cAMP-adenosine pathway is expressed in cultured bovine oviduct cells.
  • To investigate the functional relevance of this pathway in oviductal biology.

Main Methods:

  • Cultured bovine oviduct cells were treated with cyclic adenosine monophosphate (cAMP) and pathway inhibitors.
  • Inhibitors used included IBMX (phosphodiesterase inhibitor), DPSPX (ecto-phosphodiesterase inhibitor), and AMPCP (ecto-5'-nucleotidase inhibitor).

Related Experiment Videos

  • Extracellular levels of AMP, adenosine, and inosine were measured over time.

    • Main Results:

    • Exogenous cAMP increased extracellular AMP, adenosine, and inosine in a dose- and time-dependent manner.
    • Inhibitors of phosphodiesterase (PDE) and ecto-5'-nucleotidase attenuated these increases.
    • Stimulation of adenylyl cyclase led to extracellular cAMP and its conversion to adenosine.

    Conclusions:

    • The extracellular cAMP-adenosine pathway is demonstrably active in oviduct cells.
    • This pathway may significantly regulate oviduct physiology and reproductive processes.
    • Potential roles include influencing sperm function, fertilization, and early embryo development.