Related Experiment Videos
Separation and determination of phospholipids in biological samples by high-performance liquid chromatography
Fengjun Wang1, Yun Zhao, Pei Wang
1Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China. wangfj@mail.tmmu.com.cn
Abstract:
An isocratic high-performance liquid chromatographic method is developed for the determination of phospholipids in biological samples using a muPorasil silica column and a mobile phase of acetonitrile-methanol-85% phosphoric acid (90:3:1, v/v/v) at a flow rate of 0.80 mL/min. The effluent is monitored by a UV detector at 203 nm. With the method reported in this paper, phosphatidylinostol, phosphatidylserine, phosphatidylethnolamine, and phosphatidylcholine in biological samples are separated and detected successfully. The method is simple, rapid, and has excellent precision.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Elution Process
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview
There...
Thin-Layer Chromatography (TLC): Overview
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...