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Homocysteine biosynthesis pathways of Streptococcus anginosus
Yasuo Yoshida1, Masahiro Negishi, Yoshio Nakano
1Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka, Japan.
FEMS Microbiology Letters
|May 3, 2003
Abstract:
A gene (cgs) encoding cystathionine gamma-synthase was cloned from Streptococcus anginosus, and its protein was purified and characterized. The cgs gene and the immediately downstream lcd gene were shown to be cotranscribed as an operon. High-performance liquid chromatography analyses showed that the S. anginosus Cgs not only has cystathionine gamma-synthase activity, but also expresses O-acetylhomoserine sulfhydrylase activity. These results suggest that S. anginosus has the capacity to utilize both the transsulfuration and direct sulfhydrylation pathways for homocysteine biosynthesis.