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Updated: Aug 16, 2026
Stabilizing Hepatocellular Phenotype Using Optimized Synthetic Surfaces
Published on: September 26, 2014
Hepatocyte performance on different crystallographic faces of rutile
S Buchloh1, B Stieger, P J Meier
1Nonmetallic Inorganic Materials, Department of Materials, ETH Zürich, Sonneggstr. 5, 8092, Zürich, Switzerland. buchloh@nonmet.mat.ethz.ch
Abstract:
The influence of crystallographic orientation of polished rutile single crystal surfaces of the (100), (110) and (111) orientation on hepatocyte performance was tested in cell culture over 3 days. Cell adhesion was observed on the titanium dioxide surfaces and their performance was measured by means of cell number attached (protein mass), cell viability (neutral red assays) and metabolic activity (thiazolyl tetrazolium bromide assay). Titanium dioxide displays no cytotoxic effects on hepatocytes, and shows a performance in the range of standard collagen-coated tissue culture polystyrene dish. The number of hepatocytes adhered on the different rutile surfaces were not significantly different to those on dense rutile polycrystalline ceramic. These findings suggest that hepatocytes do not recognize the specific differences of differently orientated rutile crystal surfaces.
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