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Updated: Jul 11, 2026

Induction of Hypoxia in Living Frog and Zebrafish Embryos
Published on: June 26, 2017
Effects of benzodiazepines on frog ERG
1Department of Physiology, Medical Faculty, Medical University, 1431 Sofia, Bulgaria. popova@medfac.acad.bg
Abstract:
Effect of flurazepam (water-soluble benzodiazepine) on the amplitude and time course of ERG waves was investigated in superfused frog eyecups (Rana ridibunda). Flurazepam (50 and 100 microM) had inhibitory effect on the b- and d-wave amplitude, which was not accompanied with significant changes in their implicit time. Flurazepam potentiated the depressant effect of GABA (2.5 and 5 mM) on the b- and d-wave amplitude. The inhibitory effect of flurazepam was not blocked by 50 microM bicuculline (BCC), (GABA(A) antagonist), although the blocker markedly potentiated the b- and d-wave amplitude. The suppressive effect of flurazepam on the b- but not d-wave amplitude was blocked by 100 microM BCC. Our results indicate existence of functional benzodiazepine regulatory sites on GABA(A) receptors in distal frog retina.
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