The effect of storage temperature on the second day methylene-blue test

The purpose was to show whether or not the methylene-blue test can be postponed to the second day. The milk samples were preserved at three different temperatures 8-10 degrees C, 5-7 degrees C and 2-4 degrees C and the storage time was 16-22 hours. The test indicated that the methylene-blue test could be postponed to the next day satisfying the practical needs if the samples were kept at temperature of 2-4 degrees C. At this temperature the results of the second day methylene-blue test differed statistically not more than p less than 0.05 (nearly significant) from the results of the first day methylene-blue test and the percentage of quality class changes was 2.9.