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Updated: Aug 24, 2026

Analysis of Fatty Acid Content and Composition in Microalgae
Published on: October 1, 2013
Presence of free D-amino acids in microalgae
Takehiko Yokoyama1, Nobuhiro Kan-no, Takehiko Ogata
1School of Fisheries Sciences, Kitasato University, Ofunato, Iwate 022-0101, Japan.
Abstract:
Several species of microalgae (phytoplankton), 4 species of freshwater algae and 4 species of marine diatoms, were cultured germ-free in the laboratory. The presence of free D-amino acids was verified in these species by a reversed-phase HPLC analysis. D-Aspartate was detected in all the microalgae examined, but D-alanine was only present in the marine diatoms. The D-amino acid content in Asterionella sp. of the marine diatoms increased from the exponential phase to the stationary phase and then decreased to the phase of decline.
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