Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA probe attachment on plastic surfaces and microfluidic hybridization array channel devices with sample

Yingjie Liu1, Cory B Rauch

  • 1Physical Sciences Research Laboratories, Motorola Labs, Motorola Inc, 7700 South River Parkway, MD-ML34, Tempe, AZ 85284, USA. Jason_Liu@asu.edu

Analytical Biochemistry
|May 6, 2003
PubMed
Summary

The Surmodics procedure offers the most effective DNA probe immobilization on plastic surfaces for microfluidic hybridization array channel devices. This method enhances hybridization sensitivity, achieving a detection limit of 5pM with sample oscillation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High Numerical Aperture Metalens for the Deep Ultraviolet.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

The lactylation axis: bridging metabolic reprogramming and immunosuppression in the tumor microenvironment.

Frontiers in immunology·2026
Same author

Gut barrier-microbiota crosstalk in sepsis: from pathogenesis to potential therapies.

Frontiers in immunology·2026
Same author

Comparative Pharmacokinetics of Six Bioactive Constituents From Danggui Jixueteng Decoction in Normal and Myelosuppressive Rats.

Analytical science advances·2026
Same author

Loss contexts enhance dorsolateral prefrontal interpersonal neural synchrony during successful human deceptive recommendations.

Communications biology·2026
Same author

Predictive factors for remission in post-stroke depression: A Markov model cohort study.

iScience·2026

Area of Science:

  • Biotechnology
  • Materials Science
  • Analytical Chemistry

Background:

  • Fabrication of microfluidic hybridization array channel (MHAC) devices relies on effective DNA probe immobilization on plastic surfaces.
  • Optimizing plastic substrate and attachment chemistry is crucial for array performance.

Purpose of the Study:

  • To investigate and compare three oligonucleotide (oligo) probe immobilization procedures on various plastic surfaces.
  • To determine the optimal conditions for DNA probe attachment and MHAC device fabrication.
  • To enhance DNA hybridization assay sensitivity and kinetics within MHAC devices.

Main Methods:

  • Evaluated Surmodics, cetyltrimethylammonium bromide (CTAB), and Reacti-Bind procedures for oligo probe immobilization on polystyrene, polycarbonate, poly(methylmethacrylate), and polypropylene.

Related Experiment Videos

  • Assessed plastic hydrophobicity, intrinsic fluorescence, and oligo attachment efficiency.
  • Developed an improved MHAC device fabrication using CO(2) IR laser machining and demonstrated enhanced performance with sample oscillation.

    • Main Results:

    • The Surmodics procedure demonstrated superior probe spot quality and hybridization sensitivity compared to CTAB and Reacti-Bind.
    • CTAB procedure efficiency was influenced by concentration and amine-modified oligo probes.
    • MHAC devices with sample oscillation achieved a limit of detection as low as 5pM.

    Conclusions:

    • The Surmodics procedure is the most effective for DNA probe immobilization in MHAC devices.
    • Improved device fabrication and sample oscillation significantly enhance hybridization kinetics and detection sensitivity.
    • Optimized probe immobilization and device design are key for sensitive DNA detection in microfluidic arrays.