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Updated: Sep 26, 2026

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
Published on: August 6, 2019
Profilin I colocalizes with speckles and Cajal bodies: a possible role in pre-mRNA splicing
Petra Skare1, Jan-Peter Kreivi, Asa Bergström
1Department of Cell Biology, The Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden.
Abstract:
Profilin is one of the major components controlling actin polymerization. Here, profilin I was located in fibroblasts and HeLa cells by the use of two different sets of affinity-purified antibodies. Both antibody preparations labeled nuclei in a speckle-like pattern and displayed extensive colocalization with small nuclear ribonucleoprotein particle (snRNP)-core proteins and p80 coilin-containing Cajal bodies. Treatment with actinomycin D led to largely similar reorganizations of snRNPs and profilin, while profilin and Cajal bodies separated under these conditions. One of the profilin antibodies interfered with pre-mRNA splicing in vitro, further indicating a role for profilin during pre-mRNA processing.
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