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Characterization of the chlorate reductase from Pseudomonas chloritidismutans
Arthur F W M Wolterink1, Emile Schiltz, Peter-Leon Hagedoorn
1Laboratory of Microbiology, Wageningen University, 6703 CT Wageningen, The Netherlands.
Journal of Bacteriology
|May 6, 2003
Abstract:
A chlorate reductase has been purified from the chlorate-reducing strain Pseudomonas chloritidismutans. Comparison with the periplasmic (per)chlorate reductase of strain GR-1 showed that the cytoplasmic chlorate reductase of P. chloritidismutans reduced only chlorate and bromate. Differences were also found in N-terminal sequences, molecular weight, and subunit composition. Metal analysis and electron paramagnetic resonance measurements showed the presence of iron and molybdenum, which are also found in other dissimilatory oxyanion reductases.