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Controlled initiation of chromosomal replication in Escherichia coli requires functional Hda protein
Johanna Eltz Camara1, Kirsten Skarstad, Elliott Crooke
1Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20007, USA.
Journal of Bacteriology
|May 6, 2003
Abstract:
Regulatory inactivation of DnaA helps ensure that the Escherichia coli chromosome is replicated only once per cell cycle, through accelerated hydrolysis of active replication initiator ATP-DnaA to inactive ADP-DnaA. Analysis of deltahda strains revealed that the regulatory inactivation of DnaA component Hda is necessary for maintaining controlled initiation but not for cell growth or viability.