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Related Experiment Videos

C-type inactivation involves a significant decrease in the intracellular aqueous pore volume of Kv1.4 K+ channels

XueJun Jiang1, Glenna C L Bett, XiaoYan Li

  • 1Department of Physiology and Biophysics, School of Medicine and Biomedical Sciences, 124 Sherman Hall, State University of New York at Buffalo, Buffalo, NY 14214-3005, USA.

The Journal of Physiology
|May 6, 2003
PubMed
Summary

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This study measured pore volume changes in Kv1.4 K+ channels using osmotic pressure. Intracellular osmotic pressure altered channel gating kinetics, suggesting the pore closes during inactivation.

Area of Science:

  • Biophysics
  • Molecular Biology
  • Ion Channel Physiology

Background:

  • Ion channels are crucial membrane proteins that regulate ion transport.
  • Channel gating involves conformational changes affecting pore volume.
  • Understanding these volume changes is key to elucidating channel function.

Purpose of the Study:

  • To measure pore volume changes during different gating states of the Kv1.4 K+ channel (Kv1.4DeltaN).
  • To investigate the role of intracellular osmotic pressure in modulating channel gating.
  • To compare pore volume changes with known channel structures.

Main Methods:

  • Utilized giant-patch and cut-open oocyte voltage clamp techniques.
  • Applied intracellular osmotic pressure using impermeant solutes (e.g., sucrose).

Related Experiment Videos

  • Measured changes in channel activation, deactivation, inactivation, and recovery kinetics.

    • Main Results:

    • Intracellular osmotic pressure significantly altered gating kinetics: sped inactivation and deactivation, slowed activation.
    • Effects were reversible and solute-independent.
    • Estimated pore volume change during inactivation to be ~4500 A3, similar to activation/deactivation.

    Conclusions:

    • The intracellular side of the Kv1.4 K+ channel pore closes during C-type inactivation.
    • Pore volume changes during inactivation are comparable to those during activation and deactivation.
    • The C-type inactivated state shares physical similarities with the resting closed state.