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Published on: December 15, 2017
Production of native-type Streptoverticillium mobaraense transglutaminase in Corynebacterium glutamicum
Masayo Date1, Kei-ichi Yokoyama, Yukiko Umezawa
1Institute of Life Sciences, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku 210-8681, Japan.
Abstract:
We previously observed secretion of active-form transglutaminase in Corynebacterium glutamicum by coexpressing the subtilisin-like protease SAM-P45 from Streptomyces albogriseolus to process the prodomain. However, the N-terminal amino acid sequence of the transglutaminase differed from that of the native Streptoverticillium mobaraense enzyme. In the present work we have used site-directed mutagenesis to generate an optimal SAM-P45 cleavage site in the C-terminal region of the prodomain. As a result, native-type transglutaminase was secreted.
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