Related Experiment Video
Updated: Sep 26, 2026

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
Published on: March 9, 2012
Regulation of Fyn through translocation of activated Lck into lipid rafts
Dominik Filipp1, Jenny Zhang, Bernadine L Leung
1Sunnybrook and Women's College Health Sciences Centre, and Departmentof Immunology, University of Toronto, Ontario M4N 3M5, Canada.
Abstract:
Whether or how the activation of Lck and Fyn during T cell receptor (TCR) signaling is coordinated, and their delivery of function integrated, is unknown. Here we show that lipid rafts function to segregate Lck and Fyn in T cells before activation. Coaggregation of TCR and CD4 leads to Lck activation within seconds outside lipid rafts, followed by its translocation into lipid rafts and the activation of colocalized Fyn. Genetic evidence demonstrates that Fyn activation is strictly dependent on receptor-induced translocation of Lck. These results characterize the interdependence of Lck and Fyn function and establish the spatial and temporal distinctions of their roles in the cellular activation process.
Related Concept Videos
Intracellular Signaling Affects Focal Adhesions
Some...
Receptor Tyrosine Kinases
Regulation of Nuclear Protein Sorting
Mechanism of Lamellipodia Formation
Amplifying Signals via Enzymatic Cascade
The JAK-STAT Signaling Pathway

