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Updated: Jul 25, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
Published on: October 23, 2009
Electrochemical immunosensor for Forest-Spring encephalitis based on protein A labeled with colloidal gold
Khiena Brainina1, Alisa Kozitsina, Jakov Beikin
1Department of Chemistry, Ural State Economic University, 8th March Street 62, 620219, Ekaterinburg, Russia. baz@usue.ru
Abstract:
An electrochemical immunosensor for diagnosis of Forest-Spring encephalitis has been proposed. It comprises a screen-printed thick-film graphite electrode serving as the transducer and a layer of the Forest-Spring encephalitis antigen immobilized on the electrode and functioning as the biorecognition substance. The procedure includes formation of an antigen-antibody immune complex, localization of colloidal gold-labeled protein A on the complex, and recording of gold oxidation voltammogram, which provides information about the presence and the concentration of antibodies in blood serum. The response is proportional to the concentration of antibodies over the interval from 10(-7) to 10(-2) mg mL(-1). The detection limit is 10(-7) mg mL(-1).
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Enzyme-Linked Immunosorbent Assay
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