Related Experiment Videos
[Expression of pyruvate oxidase gene sopox from Streptococcus sanguis in E. coli]
1Department of Periodontology, School of Stomatology, WCUMS, Chengdu 610041, China.
Hua Xi Yi Ke Da Xue Xue Bao = Journal of West China University of Medical Sciences = Huaxi Yike Daxue Xuebao
|May 8, 2003
Abstract
Objective:
To reconstruct the expression plasmid of Sopox gene for further understanding the regulation of its expression.
Methods:
Sopox was recombined with expression vector pBV220 and the expression of Sopox in E. coli JM105 was observed after transformation.
Results:
pBV220/Sopox/JM105 expressed a protein with molecular weight of 65 kd on SDS-PAGE after induction, and the expression reached the maximal amount with induction at 42 degrees C for 4 hours.
Conclusion:
Sopox was successfully cloned into pBV220 and expressed in E. coli JM105.