Related Experiment Videos
[Effects of histones on phagocytosis and phagocytic metabolism]
Revue Canadienne De Biologie
|March 1, 1976
Abstract:
Enhanced phagocytosis and no intracellular glycogen breakdown was found in guinea-pig peritoneal polymorphonuclear leukocytes (PMN) incubated together with heat-killed staphylococci (Twort) treated with a calf thymus histone fraction (P II), for 1 hr at 37 degrees C. Glcose consumption and lactate production of these PMN's were as high as in phagocytosis of the same germ untreated with P II, and their oxygen consumption was depressed significantly.
Insights
Histone fraction P II enhanced phagocytosis in guinea pig white blood cells (PMN) without affecting intracellular glycogen breakdown. Glucose consumption and lactate production remained high, but oxygen consumption decreased.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Context:
- Investigating the role of histone fractions in immune cell function.
- Examining the interaction between bacterial components and host phagocytes.
Purpose:
- To determine the effect of calf thymus histone fraction (P II) on guinea-pig polymorphonuclear leukocytes (PMN) phagocytosis and metabolism.
- To assess intracellular glycogen breakdown, glucose consumption, lactate production, and oxygen consumption in PMN.
Summary:
- Guinea-pig peritoneal PMN incubated with heat-killed staphylococci (Twort) treated with histone fraction P II showed enhanced phagocytosis.
- No intracellular glycogen breakdown was observed in P II-treated PMN.
- Glucose consumption and lactate production were comparable to untreated controls, while oxygen consumption was significantly depressed.
Impact:
- Suggests histone fractions can modulate phagocytic activity and cellular metabolism.
- Provides insights into the biochemical mechanisms underlying immune cell responses to bacterial stimuli.
- Highlights potential therapeutic targets for modulating inflammatory responses.