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A Microfluidic System for Modeling Endothelial Dysfunction under Combined Physiological Pulsatile Shear Stress and Oscillatory Hyperglycemia
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Influences of increased oxidative stress on endothelial function, platelets function, and fibrinolysis in

Hirofumi Tomiyama1, Toshio Kushiro, Ryo Okazaki

  • 1Second Department of Internal Medicine, Tokyo Medical University, Tokyo, Japan. tomiyama@tokyo-med.ac.jp

Hypertension Research : Official Journal of the Japanese Society of Hypertension
|May 8, 2003
PubMed

Insights

Glucose intolerance worsens oxidative stress and endothelial dysfunction in hypertensive patients. Vitamin C improved endothelial function and fibrinolysis in these patients, highlighting a potential therapeutic avenue.

Area of Science:

  • Cardiovascular Medicine
  • Metabolic Disorders
  • Oxidative Stress Research

Background:

  • Endothelial, platelet, and fibrinolytic functions are crucial in atherosclerosis.
  • Glucose intolerance is known to impair endothelial function in hypertension.
  • The precise mechanisms linking glucose intolerance, oxidative stress, and endothelial dysfunction require clarification.

Purpose of the Study:

  • To investigate the impact of oxidative stress on endothelial function, platelet function, and fibrinolysis in hypertensive individuals with and without glucose intolerance.
  • To elucidate the role of glucose intolerance in exacerbating these physiological processes.
  • To assess the potential therapeutic effect of Vitamin C on these functions.

Main Methods:

  • Categorized hypertensive patients into normal glucose metabolism (n=65) and glucose intolerance (n=47) groups based on an oral glucose tolerance test.
  • Measured plasma thiobarbituric acid-reactive substances (TBARS) as a marker of oxidative stress.
  • Assessed endothelial function via flow-mediated dilatation (FMD), platelet function (EC50), and fibrinolytic parameters before and after Vitamin C administration.

Main Results:

  • Patients with glucose intolerance exhibited reduced FMD and elevated TBARS and fibrinolytic parameters compared to those with normal glucose metabolism.
  • Vitamin C significantly improved FMD and reduced fibrinolytic parameters in the glucose intolerance group, but not in the normal metabolism group.
  • Platelet function (EC50) remained similar between the groups.

Conclusions:

  • Glucose intolerance exacerbates oxidative stress, leading to impaired endothelial function in hypertensive patients.
  • These oxidative stress-induced abnormalities primarily affect fibrinolysis, not platelet function.
  • Vitamin C demonstrates a potential to ameliorate endothelial dysfunction and fibrinolytic abnormalities associated with glucose intolerance in hypertension.

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