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Related Experiment Video

Updated: Jul 9, 2026

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

G-quadruplex-specific peptide-hemicyanine ligands by partial combinatorial selection.

James A Schouten¹, Sylvain Ladame, Stephen J Mason

¹University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.

Journal of the American Chemical Society

|May 8, 2003

Summary

Researchers developed novel G-quadruplex DNA ligands by combining tetrapeptides with a hemicyanine scaffold. These ligands exhibit high affinity and selectivity for G-quadruplex DNA over duplex DNA structures.

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Last Updated: Jul 9, 2026

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Published on: March 18, 2017

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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Area of Science:

Medicinal Chemistry
Molecular Biology
Biochemistry

Background:

G-quadruplex DNA structures are unique nucleic acid motifs implicated in various biological processes.
Developing selective ligands for G-quadruplex DNA is crucial for therapeutic and diagnostic applications.
Existing ligands often lack sufficient affinity or specificity for G-quadruplex DNA.

Purpose of the Study:

To design and synthesize novel G-quadruplex DNA-specific ligands.
To enhance the binding affinity and selectivity of peptide-based ligands.
To explore the utility of hemicyanine scaffolds in G-quadruplex DNA recognition.

Main Methods:

Combinatorial selection of tetrapeptides.
Conjugation of selected tetrapeptides to a hemicyanine scaffold.
Affinity and selectivity assays for G-quadruplex DNA versus duplex DNA.

Main Results:

Generated tetrapeptide-hemicyanine conjugates as G-quadruplex DNA ligands.
Achieved an approximately 1000-fold enhancement in peptide binding affinity.
Demonstrated near micromolar affinity for G-quadruplex DNA.
Exhibited greater than 40-fold discrimination for quadruplex DNA over duplex DNA.

Conclusions:

The developed tetrapeptide-hemicyanine ligands display high affinity and selectivity for G-quadruplex DNA.
The hemicyanine scaffold significantly enhances the binding properties of the peptides.
These ligands represent promising candidates for targeting G-quadruplex DNA in biological systems.