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Dealing with different methods for Kluyveromyces lactis beta-galactosidase purification
M Becerra1, E Cerdãn, M I Gonzãlez Siso
1Departamento de Bioloxía Celular e Molecular. Facultade de Ciencias. Campus da Zapateira s/n. 15071-A Coruña. Spain. migs@udc.es
Biological Procedures Online
|May 8, 2003
Abstract:
Several micro-scale chromatography-based procedures for purification of the beta-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme.
