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Metabolic engineering to produce sesquiterpenes in yeast
Beth E Jackson1, Elizabeth A Hart-Wells, Seiichi P T Matsuda
1Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA.
Organic Letters
|May 9, 2003
Abstract:
[reaction: see text] Presented here is a metabolically engineered yeast strain that produces sesquiterpenes. Epi-cedrol synthase expressed in a native yeast strain converted endogenous farnesyl pyrophosphate to 90 microg/L epi-cedrol. This system was genetically modified to increase foreign terpene yields to 370 microg/L. The best yields were obtained by overexpressing a truncated 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in a upc2-1 mating type a background. This system allows sufficient production to characterize novel sesquiterpene synthase genes.