Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Salt-evoked lingual surface potential in humans.

George M Feldman1, Andras Mogyorosi, Gerard L Heck

  • 1Department of Medicine, Virginia Commonwealth University School of Medicine, Richmond, Virginia, 23249, USA.

Journal of Neurophysiology
|May 9, 2003
PubMed
Summary

Human salt sensing involves the epithelial sodium channel (ENaC). This study measured the lingual surface potential (LSP) in humans, finding that sodium exposure causes LSP hyperpolarization, suggesting ENaC

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential Effect of TRPV1 Modulators on Neural and Behavioral Responses to Taste Stimuli.

Nutrients·2024
Same author

Optimal Oral Iron Therapy for Iron Deficiency Anemia Among US Veterans.

JAMA network open·2024
Same author

Relationship between ENaC Regulators and SARS-CoV-2 Virus Receptor (ACE2) Expression in Cultured Adult Human Fungiform (HBO) Taste Cells.

Nutrients·2022
Same author

Interactions between Chemesthesis and Taste: Role of TRPA1 and TRPV1.

International journal of molecular sciences·2021
Same author

Mechanism of Diuresis and Natriuresis by Cannabinoids: Evidence for Inhibition of Na<sup>+</sup>-K<sup>+</sup>-ATPase in Mouse Kidney Thick Ascending Limb Tubules.

The Journal of pharmacology and experimental therapeutics·2020
Same author

Kokumi Taste Active Peptides Modulate Salt and Umami Taste.

Nutrients·2020

Area of Science:

  • Physiology
  • Neuroscience
  • Sensory Biology

Background:

  • Salt sensing in animals is mediated by the epithelial sodium channel (ENaC).
  • The potential involvement of ENaC in human salt taste perception necessitates investigation of physiological responses.
  • Lingual surface potential (LSP) changes are a potential indicator of salt sensing mechanisms.

Purpose of the Study:

  • To investigate the role of ENaC in human salt sensing by measuring the lingual surface potential (LSP).
  • To determine if sodium exposure induces measurable changes in LSP in humans.
  • To assess the effect of amiloride, an ENaC blocker, on sodium-evoked LSP changes.

Main Methods:

  • Development of a specialized chamber to measure human LSP during controlled superfusion of the tongue.

Related Experiment Videos

  • Implementation of a technique to correct for junction potentials caused by varying salt concentrations.
  • Superfusion with different solutions (e.g., NaCl, KCl) and administration of amiloride to assess LSP responses.

    • Main Results:

    • Exposure to 300 mM NaCl caused significant LSP hyperpolarization (10.1 ± 0.7 mV, P < 0.001).
    • Increased NaCl concentration led to a greater hyperpolarization (35 ± 4.8% increase from 100 to 600 mM, P < 0.001).
    • Amiloride partially inhibited the sodium-evoked LSP hyperpolarization (18.5 ± 4.3%, P < 0.005), with variable sensitivity among individuals.

    Conclusions:

    • The study successfully measured human LSP and demonstrated its hyperpolarization in response to sodium.
    • Increasing sodium concentration potentiates the LSP hyperpolarization.
    • Amiloride's inhibitory effect on sodium-evoked LSP suggests ENaC involvement, but its variable impact highlights individual differences in salt sensing.