Subcellular distribution of mouse mevalonate pyrophosphate decarboxylase

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Identification of Plasmodesmal Localization Sequences in Proteins In Planta
08:07

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Published on: August 15, 2017

Akihiro Michihara1, Kenji Akasaki, Yukio Yamori

  • 1Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan. mitihara@fupharm.fukuyama-u.ac.jp

Biological & Pharmaceutical Bulletin
|May 9, 2003
PubMed

Insights

Mevalonate pyrophosphate decarboxylase (MPD) is not upregulated by peroxisome proliferators in mice. Further studies confirm MPD is primarily located in the cytosol, not peroxisomes, in mouse melanoma cells.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Mevalonate pyrophosphate decarboxylase (MPD) has been traditionally considered a cytosolic protein.
  • Recent studies have suggested a predominant localization of MPD within peroxisomes.

Purpose of the Study:

  • To investigate the dependence of MPD expression on peroxisome proliferation in mice.
  • To determine the predominant subcellular localization of MPD in mouse tissues and cells.

Main Methods:

  • Administering peroxisome proliferative drugs to mice and analyzing liver crude extracts for MPD levels.
  • Employing cell fractionation techniques to isolate subcellular components of mouse melanoma (B16F10) cells.
  • Utilizing digitonin permeabilization to assess the cytosolic or pellet fraction distribution of MPD, lactate dehydrogenase, and catalase.

Main Results:

  • MPD protein levels in mouse liver crude extracts did not increase following administration of peroxisome proliferative drugs.
  • Cell fractionation of B16F10 cells revealed MPD exclusively in the cytosol, with no detectable presence in peroxisomes.
  • Digitonin permeabilization experiments showed that 80% of MPD was released into the medium, indicating a cytosolic localization, unlike catalase which remained in the cell pellet.

Conclusions:

  • MPD expression is independent of peroxisome proliferation and may be regulated by distinct mechanisms.
  • MPD is predominantly localized in the cytosol of mouse melanoma cells.
  • The findings challenge previous reports suggesting a primary peroxisomal localization for MPD.

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