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Updated: Aug 8, 2026

Chromosome Preparation From Cultured Cells
Published on: January 28, 2014
Chromosome 8 aneuploidy in acquired cholesteatoma
Mahmut Selman Yildirim1, Kayhan Ozturk, Hasan Acar
1Department of Genetics , Faculty of Medicine, Selcuk University, Konya, Turkey. mselman@dostmail.com
Objective:
To investigate the incidence of chromosome 8 aneuploidy in acquired cholesteatoma.
Material And Method:
Cholesteatoma tissue and postauricular skin as a control were surgically obtained from 12 patients with acquired cholesteatoma. Fluorescence in situ hybridization (FISH) analysis using a chromosome 8-specific alpha-satellite DNA probe was performed on the interphase nuclei. Two hundred cells were analyzed for each of the samples.
Results:
Chromosome 8 aneuploidy was found in 9/12 patients whereas a normal cell structure with 2 signals was observed in the remaining 3 patients. In samples with chromosome 8 aneuploidy, the mean proportion of aneuploidy was 25.6%, including monosomy (3.2%), trisomy (16.1%), tetrasomy (4.9%) and more than tetrasomy (1.4%). The number of aneuploid cells in recurrent cases was more than that in non-recurrent cases.
Conclusion:
A numerical aberration of chromosome 8 was found in patients with acquired cholesteatoma. Our results support the hypothesis that chromosome 8 may be a prognostic factor for cholesteatoma and an indicator in the follow-up of patients with cholesteatoma.
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