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The effect of body temperature on the murine electroretinogram
1Department of Ophthalmology, Columbia University, New York, NY 10032, USA.
Purpose:
To study the effect of body temperature on the murine electroretinogram (ERG).
Methods:
The corneal ERG elicited by a strobe flash from dark-adapted mice was recorded using a saline wick electrode while measuring rectal temperature continuously. The mouse was placed within a cylindrical coil of tubing through which water circulated from a temperature controlled bath. The body temperature of the mouse was changed stepwise between 30 and 37 degrees C.
Results:
ERGs of approximately normal configuration were recorded at body temperature ranging between 30 and 37 degrees C. The maximum amplitude of the a- and b-waves varied linearly with temperature. The rate of change of b-wave amplitude was about 100 microV/degree. At 30 degrees C, maximum b-wave amplitude was about 400 microV; at 37 degrees C it was about 1000 microV. A change in body temperature produced a rapid change in ERG amplitude.
Conclusion:
The murine ERG is very sensitive to changes in temperature. In order to monitor the ERG accurately over time, continuous recording of body temperature is essential.
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