Related Experiment Videos
He-Ne laser on microcrystalline arthropathies
1Facultad de Ciencias Médicas, Cátedra de Física Biomédica, Córdoba, República Argentina. campanav@hotmail.com
Objective:
The objective of this work is to assess the anti-inflammatory capacity of He-Ne laser therapy as determined by the plasmatic levels of inflammatory markers, fibrinogen, and TNFalpha and by histopathological study in rats with arthropathy induced by calcium pyrophosphate crystals.
Background Data:
Microcrystalline arthropathies are a group of diseases characterized by the deposit of different crystals in joints.
Materials And Methods:
Two milligrams of dicalcium pyrophosphate crystals (DCPP) were injected in both joints of the lower limbs of rats during 2 days. A group was treated with laser of He-Ne (6 mW) on the injected joints during 3 consecutive days. After 96 h of the first injection, animals were sacrificed to determine TNFalpha using the ELISA method and fibrinogen was assessed using spectrophotometry. Sections from the lower limbs were used for histopathology.
Results:
A statistically significant increase (p < 0.001) in plasma fibrinogen levels and TNFalpha was noted between the control group and the laser-treated group. The histological transversal section of a posterior limb joint of a rat injected with DCPP showed fibroadipose tissue with diffuse chronic infiltrate. The histopathology of the group of rats injected with DCPP and subsequently treated with He-Ne laser showed no inflammatory response.
Conclusion:
He-Ne laser treatment in the microcrystalline arthropathy induced in rats by DCPP injection might have an antiinflammatory effect, evaluated by fibrinogen plasma levels and TNF-alpha (inflammatory markers) and by the histopathology regressive process.
Insights
Helium-Neon (He-Ne) laser therapy demonstrated an anti-inflammatory effect in rats with induced arthropathy. Treatment reduced inflammatory markers like fibrinogen and TNF-alpha, and improved histopathological findings.
Area of Science:
- Biomedical Engineering
- Inflammation Research
- Laser Therapy
Background:
- Microcrystalline arthropathies involve crystal deposition in joints.
- Dicalcium pyrophosphate crystals (DCPP) can induce arthropathy.
Purpose of the Study:
- To evaluate the anti-inflammatory capacity of He-Ne laser therapy.
- To assess changes in plasma fibrinogen and TNF-alpha levels.
- To analyze histopathological alterations in induced arthropathy.
Main Methods:
- Arthropathy was induced in rats using DCPP injections.
- One group received He-Ne laser treatment on affected joints.
- Plasma inflammatory markers and joint tissues were analyzed post-treatment.
Main Results:
- He-Ne laser treatment showed a significant reduction in inflammatory markers.
- Histopathological analysis revealed no inflammatory response in treated joints.
- Laser therapy may reverse DCPP-induced arthropathy effects.
Conclusions:
- He-Ne laser therapy exhibits potential anti-inflammatory effects in DCPP-induced arthropathy.
- The study supports laser therapy as a treatment for crystal-induced joint inflammation.
- Further research is warranted to explore clinical applications.