The cagA status of Helicobacter pylori isolates from dyspeptic children in Turkey
Inci Nur Saltik1, Hülya Demir, Doruk Engin
1Department of Pediatrics, Section of Gastroenterology, Hacettepe University, Faculty of Medicine, 06100 Ankara, Turkey. isaltik@hacettepe.edu.tr
Abstract:
There are inconsistent reports regarding cytotoxin-associated gene A (cagA) status of Helicobacter pylori isolates and the severity of the mucosal lesions in children. The aim of this study was to determine the prevalence of cagA(+) strains and to evaluate its correlation with clinic and endoscopic findings. We examined 45 H. pylori strains that were grown on brain-heart infusion agar supplemented with 7% horse blood. Following 72 h of incubation colonies were harvested and bacterial DNA was extracted. Polymerase chain reaction primers F1 and B1 were used to amplify a 348-bp internal fragment of cagA. The prevalence of cagA in Turkish pediatric patients was 55.6%. No association was found between cagA status and the severity of gastro-duodenal lesions.
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