Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution.

Camilla O Samuelsen1, Vera Baraznenok, Olga Khorosjutina

  • 1Department of Genetics, Institute of Molecular Biology, Oester Farimagsgade 2A, DK-1353 Copenhagen, Denmark.

Proceedings of the National Academy of Sciences of the United States of America
|May 10, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteolytic cleavage activates the mitochondrial isoform of TOP3A.

Nucleic acids research·2025
Same author

An injury-associated lobular microniche is associated with the classical tumor cell phenotype in pancreatic cancer.

Nature communications·2025
Same author

SMAD3 and p300 complex scaffolding by long non-coding RNA LIMD1-AS1 promotes TGF-β-induced breast cancer cell plasticity.

Nucleic acids research·2025
Same author

Expanding the Genetic and Phenotypic Spectrum of POLRMT-Related Mitochondrial Disease.

Clinical genetics·2025
Same author

In vivo composition of the mitochondrial nucleoid in mice.

Biochimica et biophysica acta. Molecular cell research·2025
Same author

Small molecules restore mutant mitochondrial DNA polymerase activity.

Nature·2025

A conserved Mediator module, including Srb8/TRAP230/ARC240 and Srb9/TRAP240/ARC250, negatively regulates transcription across eukaryotes. This module, when present, prevents RNA polymerase II association with Mediator.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The Mediator complex plays a crucial role in eukaryotic transcription regulation.
  • A specific module (Srb8, Srb9, Srb10, Srb11) in yeast Mediator is linked to negative transcriptional regulation.
  • Homologues for Srb10 and Srb11 exist in mammals, but Srb8 and Srb9 homologues were less clear.

Purpose of the Study:

  • To identify and characterize Srb8 and Srb9 homologues in Schizosaccharomyces pombe.
  • To investigate the association of these homologues with the Mediator complex and RNA polymerase II.
  • To determine the functional conservation of this Mediator module across eukaryotes.

Main Methods:

  • Identification of a TRAP240/ARC250 homologue (spTrap240) in Schizosaccharomyces pombe.

Related Experiment Videos

  • Biochemical isolation of Mediator complexes with and without the spSrb8/spTrap240 module.
  • Analysis of gene expression and phenotypic characteristics regulated by spTrap240 and spSrb8.
  • Assessment of RNA polymerase II association with different Mediator forms.

    • Main Results:

    • A conserved Mediator module containing spSrb8, spTrap240, spSrb10, and spSrb11 was identified in Schizosaccharomyces pombe.
    • spTrap240 and spSrb8 regulate a distinct gene subset and exhibit similar phenotypes.
    • Mediator complexes containing this module are isolated free of RNA polymerase II.
    • Mediator complexes lacking this module associate with RNA polymerase II.

    Conclusions:

    • TRAP230/ARC240 and TRAP240/ARC250 are likely the mammalian Srb8 and Srb9 homologues, respectively.
    • The Srb8/TRAP230/ARC240, Srb9/TRAP240/ARC250, Srb10, and Srb11 module is conserved in eukaryotes.
    • This conserved module negatively regulates transcription by preventing RNA polymerase II recruitment.