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Related Experiment Videos

A standard for human plasmin.

T B Kirkwood, P J Campbell, P J Gaffney

    Thrombosis Et Diathesis Haemorrhagica
    |September 30, 1975
    PubMed
    Summary
    This summary is machine-generated.

    A human plasmin standard (72/379) in glycerol is suitable for bioassaying plasmin activity. This stable preparation is recommended for defining plasmin units in laboratory settings.

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    Area of Science:

    • Biochemistry
    • Hematology
    • Clinical Chemistry

    Background:

    • Accurate measurement of plasmin activity is crucial for understanding fibrinolysis and related disorders.
    • A standardized reference material is needed for reliable bioassays of plasmin activity across different laboratories.

    Purpose of the Study:

    • To evaluate a human plasmin preparation (72/379) as a single standard for plasmin activity bioassays.
    • To assess the stability and suitability of the proposed plasmin standard.

    Main Methods:

    • An international collaborative study involving ten laboratories.
    • Evaluation of the stability of the human plasmin preparation in 50% glycerol.
    • Comparison of different bioassay methods for plasmin activity.

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    Main Results:

    • The human plasmin preparation (72/379) demonstrated suitability as a standard.
    • The preparation exhibits sufficient stability for defining plasmin units at 4°C for extended periods and at 20°C for short durations.
    • A potency of 8 units/ml was assigned to the standard.

    Conclusions:

    • The human plasmin preparation 72/379 is recommended as a standard for plasmin activity bioassays.
    • This standard is stable and suitable for use in clinical and research settings.
    • International bodies have endorsed the use of this material as a reference standard.