Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multienzyme catalysis in microfluidic biochips.

Moo-Yeal Lee1, Aravind Srinivasan, Bosung Ku

  • 1Department of Chemical Engineering, Rensselaer Polytechnic Institute, 103 Ricketts Building, Troy, New York 12180, USA.

Biotechnology and Bioengineering
|May 13, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic pillar-perfusion platform for screening enzyme-induced self-assembling peptide therapeutics in 3D breast cancer spheroids.

Bioengineering & translational medicine·2026
Same author

Evaluation of <i>in vitro</i> blood-brain barrier models in central nervous system new drug development: methodological challenges and translational perspectives.

Expert opinion on drug delivery·2026
Same author

Multicenter Clinical Comparison of the 10-Minute AMDI Fast PCR Mini Respiratory Panel and the Cepheid Xpert Xpress CoV-2/Flu/RSV plus.

The Journal of molecular diagnostics : JMD·2026
Same author

Circadian disruption induces sex-specific Alzheimer's pathophysiology and immune cell reprogramming.

bioRxiv : the preprint server for biology·2026
Same author

Effect of transcript length, temperature, and lyophilization on mRNA-lipid nanoparticle stability.

Journal of pharmaceutical sciences·2026
Same author

Analytical performance of the AMDI™ Fast PCR Mini Respiratory Panel.

Diagnostic microbiology and infectious disease·2026
Same journal

Developing Anti-EGFR/Anti-HER2 Bifunctional Antibody for Solid Tumors by Protein Engineering.

Biotechnology and bioengineering·2026
Same journal

Bridging Organ-on-a-Chip and Omics: A Multi-Dimensional Frontier in Biomedical Research.

Biotechnology and bioengineering·2026
Same journal

Hemopexin Purification From Human Cohn Fraction IV Paste and Its Biophysical Characterization and Functional Evaluation in Sickle Cell Disease Mice.

Biotechnology and bioengineering·2026
Same journal

Characterization and Therapeutic Potential of a Novel Lytic Phage-Derived Endolysin PA16cLys Against Uropathogenic Pseudomonas aeruginosa Biofilms.

Biotechnology and bioengineering·2026
Same journal

Modeling Multiscale Architecture of Biofilm Extracellular Matrix and Its Role in Oxygen Transport.

Biotechnology and bioengineering·2026
Same journal

A Behavior-Integrated Potency Assay for Quantitative Evaluation of Extracellular Matrix Remodeling by Mesenchymal Stem/Stromal Cells.

Biotechnology and bioengineering·2026
See all related articles

Enzyme immobilization on glass microfluidic channels was achieved using a poly(maleic anhydride-alt-alpha-olefin) (PMA) coating. This method preserves enzyme activity, enabling efficient microscale biocatalysis and multi-enzyme reactions.

Area of Science:

  • Biocatalysis
  • Microfluidics
  • Surface Chemistry

Background:

  • Enzyme immobilization is crucial for biocatalysis, but maintaining native activity in microfluidic systems remains challenging.
  • Developing mild and effective immobilization strategies is essential for microscale enzyme applications.

Purpose of the Study:

  • To develop a novel method for enzyme immobilization onto glass microfluidic channels.
  • To investigate the activity and kinetics of immobilized enzymes at the microscale.
  • To demonstrate the feasibility of multi-enzyme reactions in microfluidic devices.

Main Methods:

  • Utilized a poly(maleic anhydride-alt-alpha-olefin) (PMA) coating for enzyme attachment to glass microfluidic channels.
  • Immobilized soybean peroxidase (SBP) using a mixed covalent-noncovalent approach.

Related Experiment Videos

  • Performed kinetic studies using Michaelis-Menten analysis and demonstrated bienzymic and trienzymic reactions.

    • Main Results:

    • Achieved efficient enzyme immobilization with up to 0.6 microg SBP per channel.
    • Immobilized SBP exhibited native Michaelis-Menten kinetics, comparable to free enzymes.
    • Successfully demonstrated bienzymic and trienzymic reactions, including the synthesis of poly(p-cresol).

    Conclusions:

    • The PMA-based coating provides a mild and effective method for enzyme immobilization in microfluidic channels.
    • Enzymes immobilized using this technique retain their native activity at the microscale.
    • This approach enables the development of microfluidic biochips for complex biocatalytic processes.