Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzyme-containing Michael-adduct-based coatings.

Géraldine F Drevon1, Claus Urbanke, Alan J Russell

  • 1Department of Chemical and Petroleum Engineering, University of Pittsburgh, 1249 Benedum Hall, Pittsburgh, Pennsylvania 15261, USA.

Biomacromolecules
|May 14, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human Disposition, Metabolism, and Excretion of Sevasemten (EDG-5506), a Selective Modulator of Fast Myosin in Healthy Volunteers.

Journal of clinical pharmacology·2026
Same author

Effects of sevasemten (EDG-5506) on safety, biomarkers, and functional measures in adults with Becker muscular dystrophy: results of a phase 1b, open-label study.

EBioMedicine·2026
Same author

Exploring the evidence for the use of protein biomarkers of muscular damage and disease progression in Duchenne and Becker muscular dystrophy: a systematic review and meta-analysis protocol.

BMJ open·2025
Same author

Short-term daily deflazacort decreases membrane permeability and increases maximum force in <i>mdx</i> mice.

American journal of physiology. Cell physiology·2025
Same author

Versatile roles of rubredoxin reductase of Pseudomonas aeruginosa TBCF10839 in virulence and stress protection.

PLoS pathogens·2025
Same author

Heterozygous <i>MYH7</i> R403Q mutation impairs left atrial mitochondrial function in a Yucatan mini-pig model of genetic nonobstructive hypertrophic cardiomyopathy.

Journal of applied physiology (Bethesda, Md. : 1985)·2025
Same journal

Metal-Ion-Modulated Stabilization of Intermediates in Diphenylalanine and Phenylalanine Self-Assembly: Roles of Metal-Ion-Specific Salt Bridges and Hydrophobic Interactions.

Biomacromolecules·2026
Same journal

Understanding Processing-Structure-Property Relationships in Spun Spidroin-Mimetic Fibers Using Molecular Dynamics Simulation.

Biomacromolecules·2026
Same journal

Exploring the Antibacterial Potential of Bis-MPA Dendrimers Bearing Cu(II) and Zn(II) Complexes.

Biomacromolecules·2026
Same journal

Toward Sustainable Optics: Fully Biomass-Based Vitrimer Film for Near-Infrared Transparency.

Biomacromolecules·2026
Same journal

Poly(furfuryl alcohol) as a Surface Modifier for Cellulose Nanocrystals Reinforced HDPE Nanocomposites.

Biomacromolecules·2026
Same journal

Cutinase Adsorption to Polyester Surfaces Modulates Dissolved Oligomer Speciation during Enzymatic Depolymerization.

Biomacromolecules·2026
See all related articles

A novel two-step method efficiently immobilizes carbonic anhydrase (CA) into coatings, enhancing enzyme stability and activity for industrial applications. This method offers superior performance compared to other immobilization techniques.

Area of Science:

  • Biocatalysis
  • Materials Science
  • Enzyme Immobilization

Background:

  • Carbonic anhydrase (CA) is a crucial enzyme with broad applications.
  • Efficient immobilization of CA into coatings is challenging.
  • Existing methods often result in reduced enzyme activity and stability.

Purpose of the Study:

  • To develop a homogeneous and efficient method for immobilizing carbonic anhydrase (CA) into Michael-adduct-based coatings.
  • To compare the developed method with existing two-step and three-step immobilization techniques.
  • To evaluate the activity and stability of the immobilized enzyme-containing coatings (ECC).

Main Methods:

  • A two-step method involving covalent coupling of CA to an N-vinylformamide-based water-soluble polymer.

Related Experiment Videos

  • Dispersion of the polymer/CA conjugate within a film matrix to form enzyme-containing coatings (ECC).
  • Comparison with alternative two-step surface coupling and three-step immobilization processes.

    • Main Results:

    • The developed two-step method achieved homogeneous insertion of CA into coatings.
    • The resulting ECC retained approximately 7% apparent activity in hydrolyzing p-nitrophenyl propionate.
    • This method demonstrated higher efficiency and retained more activity compared to other tested techniques.
    • The CA-ECC exhibited good stability, retaining 45% activity after 90 days of storage at room temperature.

    Conclusions:

    • The developed two-step homogeneous immobilization method is superior for incorporating carbonic anhydrase into coatings.
    • This technique enhances enzyme stability and preserves significant catalytic activity.
    • The resulting enzyme-containing coatings show promise for various applications requiring stable and active biocatalysts.