Related Experiment Video
Updated: Jul 11, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Biotransformation of limonene by bacteria, fungi, yeasts, and plants
W A Duetz1, H Bouwmeester, J B van Beilen
1Institute of Biotechnology, ETH Hönggerberg, HPT, 8093 Zürich, Switzerland. duetz@enzyscreen.com
Abstract:
The past 5 years have seen significant progress in the field of limonene biotransformation, especially with regard to the regiospecificity of microbial biocatalysts. Whereas earlier only regiospecific biocatalysts for the 1,2 position (limonene-1,2-diol) and the 8-position (alpha-terpineol) were available, recent reports describe microbial biocatalysts specifically hydroxylating the 3-position (isopiperitenol), 6-position (carveol and carvone), and 7-position (perillyl alcohol, perillylaaldehyde, and perillic acid). The present review also includes the considerable progress made in the characterization of plant P-450 limonene hydroxylases and the cloning of the encoding genes.
Related Concept Videos
Bioremediation
Drug Biotransformation: Overview
Drug Biotransformation: Overview
Environmental Applications of Microorganisms
Microbial Bioremediation of Pesticides
Microbial Leaching

