Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Acute lymphoblastic leukemia: background.

John Swansbury¹

¹Academic Haematology and Cytogenetics, The Royal Marsden NHS Trust, The Institute of Cancer Research, Surrey, UK.

Methods in Molecular Biology (Clifton, N.J.)

|May 15, 2003

Summary

Cytogenetic analysis in acute lymphoblastic leukemia (ALL) presents challenges but offers crucial diagnostic information. This book explores practical approaches from leading labs to overcome technical difficulties and improve success rates in ALL cytogenetics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Clonal diversity of MYC amplification evaluated by fluorescent in situ hybridisation and digital droplet polymerase chain reaction in oesophagogastric cancer: Results from a prospective clinical trial screening programme.

European journal of cancer (Oxford, England : 1990)·2019

Same author

Desmoplastic small round cell tumor: evaluation of reverse transcription-polymerase chain reaction and fluorescence in situ hybridization as ancillary molecular diagnostic techniques.

Virchows Archiv : an international journal of pathology·2017

Same author

The spectrum of EWSR1-rearranged neoplasms at a tertiary sarcoma centre; assessing 772 tumour specimens and the value of current ancillary molecular diagnostic modalities.

British journal of cancer·2017

Same author

Recurrent Cytogenetic Abnormalities in Myeloproliferative Neoplasms and Chronic Myeloid Leukemia.

Methods in molecular biology (Clifton, N.J.)·2016

Same author

The distinctive micromegakaryocytes of transformed chronic myeloid leukemia.

American journal of hematology·2016

Same author

Ancillary molecular analysis in the diagnosis of soft tissue tumours: reassessment of its utility at a specialist centre.

Journal of clinical pathology·2015

Area of Science:

Cytogenetics
Hematologic Malignancies
Molecular Diagnostics

Background:

Acute lymphoblastic leukemia (ALL) is a hematologic malignancy where cytogenetic analysis is vital for diagnosis and prognosis.
Cytogenetic studies in ALL face technical hurdles including sample clotting, poor chromosome morphology, and low cell division rates.

Purpose of the Study:

To present practical strategies for overcoming common technical challenges in cytogenetic analysis of ALL.
To illustrate contrasting successful approaches from different high-achieving laboratories.

Main Methods:

The book details two distinct laboratory approaches to cytogenetic studies in ALL.
Emphasis is placed on practical aspects and successful methodologies for sample processing and analysis.

Main Results:

Both presented methodologies demonstrate high success rates in obtaining diagnostic cytogenetic information from ALL samples.
The approaches are adaptable, offering valuable insights for laboratories with varying workloads and sample sizes.

Conclusions:

Effective cytogenetic analysis in ALL is achievable despite technical difficulties through optimized laboratory practices.
Emulation of successful strategies can enhance diagnostic capabilities for acute lymphoblastic leukemia.

Related Experiment Videos