Related Experiment Video
Updated: Sep 26, 2026

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
Published on: May 24, 2017
Chemical cleavage reactions of DNA on solid support: application in mutation detection
Chinh T Bui1, Andreana Lambrinakos, Jeffrey J Babon
1Genomic Disorders Research Centre (the University of Melbourne) 7th Floor, Daly Wing, St, Vincent's Hospital, 35 Victoria parade, Fitzroy, Vic, 3065, Australia. chinhbui@mail.medstv.unimelb.edu.au
Abstract:
BACKGROUND: The conventional solution-phase Chemical Cleavage of Mismatch (CCM) method is time-consuming, as the protocol requires purification of DNA after each reaction step. This paper describes a new version of CCM to overcome this problem by immobilizing DNA on silica solid supports. RESULTS: DNA test samples were loaded on to silica beads and the DNA bound to the solid supports underwent chemical modification reactions with KMnO4 (potassium permanganate) and hydroxylamine in 3M TEAC (tetraethylammonium chloride) solution. The resulting modified DNA was then simultaneously cleaved by piperidine and removed from the solid supports to afford DNA fragments without the requirement of DNA purification between reaction steps. CONCLUSIONS: The new solid-phase version of CCM is a fast, cost-effective and sensitive method for detection of mismatches and mutations.
Related Concept Videos
DNA Isolation
Overview of DNA Repair
Chemically...
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Spontaneous and Induced Mutations
Mismatch Repair
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...

