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Genetic immunization is augmented by murine polyomavirus VP1 pseudocapsids
E Rollman1, T Ramqvist, B Zuber
1Department of Virology, Swedish Institute for Infectious Disease Control and the Microbiology and Tumor Biology Center, Karolinska Institute, SE-171 82, Solna, Sweden. erik.rollman@smi.ki.se
Abstract:
To improve immune responses induced by DNA immunization, murine polyomavirus major capsid protein (VP1) pseudocapsids were complexed with a DNA plasmid encoding the p37 (p24 and p17) nucleocapsid proteins of the human immunodeficiency virus type 1 (HIV-1). A 10-fold increase in antibody titer was noted in mice given DNA plasmid together with VP1 pseudocapsids in comparison to animals that received DNA plasmid alone. Cell mediated responses to HIV-1 p24 occurred, but were not significantly augmented by delivering the DNA as a VP1 complex. We have consequently for the first time shown a carrier/adjuvant effect of polyomavirus pseudocapsids that strongly increased the humoral immune response in DNA immunization.