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Related Experiment Videos

GLUT12 expression in human placenta in first trimester and term.

N M Gude1, J L Stevenson, S Rogers

  • 1Department of Perinatal Medicine, Royal Women's Hospital, Carlton, Victoria, Australia.

Placenta
|May 15, 2003
PubMed
Summary

This study investigated the novel glucose transporter GLUT12 in human placenta. GLUT12 expression changes significantly from early pregnancy to term, indicating a dynamic role in placental function.

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Area of Science:

  • Obstetrics and Gynecology
  • Cell Biology
  • Molecular Biology

Background:

  • Glucose transporters are crucial for nutrient supply to the fetus.
  • The role of glucose transporter 12 (GLUT12) in placental development is largely unknown.
  • Understanding placental nutrient transport is vital for fetal growth and health.

Purpose of the Study:

  • To characterize the spatiotemporal expression pattern of the novel glucose transporter GLUT12 in the human placenta.
  • To investigate GLUT12 mRNA and protein localization during different stages of pregnancy.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect GLUT12 mRNA in placental tissue and cell lines.
  • Immunohistochemistry with a GLUT12 specific antibody was performed on first-trimester and term placental tissues.

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  • Analysis included chorionic villus sampling and term placental tissue.

    • Main Results:

    • GLUT12 mRNA was detected in term placentae and placental cell lines (JAR, JEG-3, HTR-8Svneo).
    • In first-trimester placenta (10-12 weeks), GLUT12 protein was localized to the syncytiotrophoblast and extra-villous trophoblast.
    • In term placenta, GLUT12 protein was absent in the syncytiotrophoblast but present in villous vascular smooth muscle and stromal cells.

    Conclusions:

    • Human placental expression of GLUT12 exhibits dynamic spatial and temporal changes throughout gestation.
    • GLUT12's changing localization suggests a potentially evolving role in placental glucose transport and function.
    • Further research is warranted to elucidate the specific functions of GLUT12 in placental physiology.