Related Experiment Video
Updated: Aug 8, 2026
11:00
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
Published on: November 9, 2017
Cytokine flow cytometry: multiparametric approach to immune function analysis
1BD Biosciences, Immunocytometry Systems, San Jose, CA 95131, USA.
Cytotherapy
|May 15, 2003
Summary
Cytokine flow cytometry offers precise cellular immune response quantitation. New automated methods promise to reveal protection correlates in cellular immunity diseases through large clinical trials.
Area of Science:
- Immunology
- Cellular Immunology
- Immune Monitoring
Background:
- Precise quantitation of cellular immune responses is crucial for understanding immunity.
- Single-cell assays like cytokine flow cytometry, ELISPOT, and MHC-peptide multimers have advanced immune response measurement.
- Cytokine flow cytometry allows MHC-unrestricted antigen response detection and multiparameter cell analysis.
Purpose of the Study:
- To compare cytokine flow cytometry with other immune function assays.
- To summarize data from cytokine flow cytometry in various disease states.
- To describe methodological improvements for cytokine flow cytometry.
Main Methods:
- Review and comparison of immune function assays.
- Summary of existing cytokine flow cytometry data in disease.
- Description of technical advancements in cytokine flow cytometry.
Main Results:
- Cytokine flow cytometry provides detailed cellular immune response data.
- Methodological improvements enhance assay robustness, throughput, and information content.
- The technique is applicable across diverse disease states.
Conclusions:
- Cytokine flow cytometry is a powerful tool for immune response analysis.
- Advancements are making the technique more efficient and informative.
- Automated cytokine flow cytometry may elucidate correlates of protection in cellular immunity diseases.
