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A novel method of generating prostate cancer metastases from orthotopic implants
Eva Corey1, Janna E Quinn, Robert L Vessella
1University of Washington, Department of Urology, Seattle, Washington 98195, USA. ecorey@u.washington.edu
Background:
Spontaneous metastasis following implantation at the orthotopic site is a highly desired feature in prostate cancer (CaP) models, since it would enable studies of mechanisms associated with tumor cell dissemination.
Methods:
LuCaP 23.8 and LuCaP 35, hormone-sensitive CaP xenografts that express PSA and the wild-type androgen receptor, were grown orthotopically in SCID mice. When tumor volumes reached approximately 250-500 mg, primary tumors were removed to allow micrometastases to grow.
Results:
Using this procedure we generated macroscopic metastases (>20 mg) in 71% (LuCaP 23.8) and 100% (LuCaP 35) of animals, respectively.
Conclusions:
These models may be used to evaluate new treatment modalities and study mechanisms associated with development of metastases.