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Proteomic analysis of human prostasomes
Angelita G Utleg1, Eugene C Yi, Tao Xie
1Institute for Systems Biology, Seattle, Washington 98103, USA.
The Prostate
|May 15, 2003
Summary
This study identified 139 proteins in prostasomes, with most previously undescribed. This proteome dataset offers a valuable reference for future research on prostate health and disease.
Area of Science:
- Biochemistry
- Proteomics
- Human Seminal Fluid Analysis
Background:
- Prostasomes are poorly understood secretory particles found in human seminal fluid.
- Previous research on prostasome protein composition was limited to basic descriptions and incomplete 2D gel electrophoresis (2DE) studies.
Purpose of the Study:
- To comprehensively catalogue the protein composition of human prostasomes.
- To establish a foundational dataset for comparative studies of prostasomes in health and disease.
Main Methods:
- Utilized Gas phase fractionation coupled with microcapillary HPLC-tandem mass spectrometry (microLC-MS/MS).
- Employed a direct iterative approach for proteome cataloguing.
Main Results:
- Identified 139 distinct proteins within prostasomes.
- Categorized identified proteins into enzymes (33.8%), transport/structural (19.4%), GTP proteins (14.4%), signal transduction (17.3%), chaperones (5.8%), and unannotated (9.4%).
- Found that 128 out of 139 identified proteins were novel to prostasomal composition.
Conclusions:
- The identified prostasome proteome serves as a critical reference dataset.
- This dataset will facilitate future comparative analyses of prostasomes in conditions including prostate cancer, benign prostatic hyperplasia, prostatitis, and infertility.