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Genotoxic effects of PAH containing sludge extracts in Chinese hamster ovary cell cultures
K Krishnamurthi1, Font Devi, T Chakrabarti
1Environmental Biotechnology Division, National Environmental Engineering Research Institute, Nehru Marg, Nagpur-440 020, India.
Objective:
Many studies have been conducted in order to evaluate the genotoxicity of chemicals and waste materials, which utilized in vivo test protocols. The use of animals for routine toxicity testing is now questioned by a growing segment of society.
Methods:
Keeping the above fact in mind, we have conducted in the present study the genotoxicity evaluation of oily sludge samples generated from a petroleum refinery and petrochemical industry and ETP sludge from petroleum refinery using DNA damage, chromosomal aberration, p53 protein induction and apoptosis in short term in vitro mammalian Chinese Hamster Ovary cell cultures.
Results:
It is evident from the results that the oily sludge compounds derived from petroleum refinery and petrochemical industry could cause DNA damage, chromosomal aberration, p53 protein accumulation and apoptotic cell death on exposure to oily sludge extracts in the presence of metabolic activation system (S-9 mix), however, ETP sludge extract could not cause significant genotoxicity in comparison to oily sludge extract and negative control.
Conclusion:
The effect may be attributed to polycyclic aromatic hydrocarbons present in the samples as evidenced from GC-MS.
