Related Experiment Video

Updated: Sep 26, 2026

Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex
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Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex

Published on: August 2, 2021

Lens epithelial cells express CD95 and CD95 ligand treatment induces cell death and DNA fragmentation in vitro

A Hueber1, C D Eichholtz, N Kociok

  • 1Center of Ophthalmology, University of Cologne, Cologne, Germany. Arno.Hueber@Uni-Koeln.de

European Journal of Ophthalmology
|May 16, 2003
PubMed
Abstract

Insights

CD95 ligand (CD95L) induces apoptosis in lens epithelial cells (LEC), offering a potential strategy to inhibit proliferation and prevent posterior capsule opacification after cataract surgery.

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Molecular Biology

Background:

  • Posterior capsule opacification (PCO) is a common complication following cataract surgery.
  • Antiproliferative agents are explored to mitigate PCO.
  • CD95 ligand (CD95L) induces apoptosis, a programmed cell death mechanism.

Purpose of the Study:

  • To characterize CD95L-induced apoptosis in cultured lens epithelial cells (LEC).
  • To evaluate the potential of CD95L as an antiproliferative agent for preventing PCO.

Main Methods:

  • Porcine LEC were analyzed for CD95 expression using flow cytometry.
  • Cell death was quantified via crystal violet assay.
  • DNA fragmentation was measured using the comet assay.

Main Results:

  • Lens epithelial cells (LEC) express CD95.
  • CD95L treatment caused time- and concentration-dependent cell death in LEC.
  • Apoptosis was confirmed by DNA fragmentation, with synergistic enhancement by cyclohexamide.

Conclusions:

  • Apoptosis-inducing CD95L effectively inhibits LEC proliferation.
  • Further research is required for the clinical application of CD95L to prevent PCO.

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