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Related Experiment Videos

Estrogen increases inducible nitric oxide synthase gene expression.

Leigh S Walsh1, Arthur Ollendorff, John L Mershon

  • 1Department of Obstetrics and Gynecology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

American Journal of Obstetrics and Gynecology
|May 16, 2003
PubMed
Summary

Estrogen significantly boosts inducible nitric oxide synthase (iNOS) gene expression in cells with estrogen receptors. This finding supports estrogen's inflammatory role and cardiovascular impact.

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Area of Science:

  • Endocrinology
  • Molecular Biology
  • Genetics

Background:

  • Estrogen is a key hormone with diverse physiological roles.
  • Nitric oxide synthases (NOS) are crucial enzymes involved in various biological processes.
  • Inducible nitric oxide synthase (iNOS) plays a role in inflammation and immune responses.

Purpose of the Study:

  • To investigate the effect of estrogen on the expression of the inducible nitric oxide synthase (iNOS) gene.
  • To elucidate the role of estrogen receptors in mediating iNOS gene regulation.

Main Methods:

  • Constructed an iNOS fusion gene linked to the luciferase reporter gene.
  • Transiently transfected COS cells with the fusion gene and estrogen receptor-alpha expression plasmid.
  • Exposed cells to estradiol or a cytokine mix and measured gene expression.

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Main Results:

  • Estradiol significantly increased iNOS gene expression by 31.2% in cells with estrogen receptors (P =.006).
  • Cells lacking estrogen receptors showed no significant increase in iNOS expression with estradiol exposure.

Conclusions:

  • Estrogen administration upregulates inducible nitric oxide synthase expression.
  • These findings suggest a mechanism for estrogen's inflammatory effects and potential cardiovascular implications.