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ProteomeWeb: a web-based interface for the display and interrogation of proteomes.

György Babnigg1, Carol S Giometti

  • 1Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, USA. gbabnigg@anl.gov

Proteomics
|May 16, 2003
PubMed
Summary

This study presents a web-based tool for analyzing microbial proteomes. It integrates protein expression data with genomic and metabolic pathway information for efficient analysis.

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Area of Science:

  • Proteomics
  • Bioinformatics
  • Genomics

Background:

  • Proteome analysis involves separating, quantifying, and identifying proteins.
  • Diverse data formats are generated, requiring integration.
  • Microbial proteome characterization is crucial for research.

Purpose of the Study:

  • To develop a World-Wide Web-based interface for displaying and analyzing proteomic data.
  • To integrate proteome analysis with genomic and metabolic pathway information.
  • To expedite the analysis of microbial protein expression profiles.

Main Methods:

  • Development of a web-based interface for two-dimensional gel electrophoresis (2D-PAGE) protein profiles.
  • On-the-fly protein identification and quantitative data interrogation.

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  • Integration with genome sequence information and metabolic pathway databases.

    • Main Results:

    • The web interface facilitates the display and analysis of proteomic data.
    • Efficient determination of gene locations for abundance-modulated proteins.
    • Relocation of proteins within specific metabolic pathways is enabled.

    Conclusions:

    • The developed web interface enhances proteome analysis capabilities.
    • It provides a valuable tool for studying microbial proteomes, exemplified by Methanococcus jannaschii.
    • The interface supports efficient data integration and interpretation for biological insights.