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Aflatoxin binders I: in vitro binding assay for aflatoxin B1 by several potential sequestering agents
Duarte E Diaz1, Winston M Hagler, Brinton A Hopkins
1Department of Animal Science, College of Agriculture and Life Sciences, North Carolina State University, Raleigh, NC 27695-7621, USA.
Mycopathologia
|May 17, 2003
Summary
Nine sequestering agents, including activated charcoals and bentonites, effectively bind aflatoxin B1 (AFB1) in vitro. These agents show potential for use in animal feed to mitigate AFB1 contamination across various gastrointestinal pH levels.
Area of Science:
- Animal Science
- Food Science
- Toxicology
Background:
- Aflatoxin B1 (AFB1) is a toxic mold metabolite posing risks to animal health and food safety.
- Effective sequestering agents are needed to mitigate AFB1 contamination in animal feed.
Purpose of the Study:
- To evaluate the in vitro efficacy of nine proprietary sequestering agents for binding AFB1.
- To assess the binding capacity of agents across a range of pH conditions relevant to animal digestion.
Main Methods:
- A novel in vitro assay was developed to test AFB1 binding.
- Four activated charcoals, three sodium bentonites, one calcium bentonite, and one esterified glucomannan were evaluated.
- Agents were tested in 10% methanol suspensions at pH 3, 7, 10, and pH-unadjusted with AFB1 at 5 µg/ml.
Main Results:
- All nine agents demonstrated high AFB1 binding efficiency, exceeding 95%.
- Activated charcoals achieved over 99% binding, sodium bentonites bound 95-98%, calcium bentonite bound 98%, and esterified glucomannan bound 97%.
- Effective binding was observed across all tested pH values.
Conclusions:
- The tested sequestering agents exhibit significant potential for binding AFB1 in conditions mimicking ruminant gastrointestinal tracts.
- These agents could serve as valuable tools for reducing AFB1 exposure in livestock.
- Further in vivo studies are warranted to confirm efficacy in animal systems.