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Lipase-catalysed transesterification of soya bean oil for biodiesel production during continuous batch operation
Wei Du1, Yuanyuan Xu, Dehua Liu
1Department of Chemical Engineering, Tsinghua University, Beijing 100084, People's Republic of China. duwei@tsinghua.edu.cn
Biotechnology and Applied Biochemistry
|May 17, 2003
Abstract:
The effects of temperature, oil/alcohol molar ratio and by-product glycerol were studied during Lipozyme TL IM-catalysed continuous batch operation when short-chain alcohols were used as the acyl acceptor. In non-continuous batch operation, the optimal oil/alcohol ratio and temperature were 1:4 and 40-50 degrees C; however, during the continuous batch operation, the optimal oil/alcohol ratio and temperature were 1:1 and 30 degrees C; 95% of enzymic activity remained after 10 batches when isopropanol was adopted to remove by-product glycerol during repeated use of the lipase.