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Fibrin powder turbidity measurement for rapid assessment of antiplasmins
Abstract:
Fibrinolytic activity was determined from the rate of disappearance of turbidity in a suspension of heat-treated fibrin powder. Using this method for estimating residual fibrinolytic activity in mixtures of serum and plasmin, antiplasmin behaviors of specimens from patients with various clinical disorders were determined after long and short preincubation times. Slow-acting antiplasmins were found to be increased in a variety of conditions among these patients, while immediate acting antiplasmins were generally decreased, compared with those in specimens from a large pool of normal, healthy vounteers. Normal women taking oral contraceptives had consitently high levels of slow antiplasmins. Tests in vitro showed that the antifibrinolytic agents epsilon-aminocaproic acid, Trasylol and soybean trypsin inhibitor act only as fast antiplasmins.
Insights
This study reveals that slow-acting antiplasmins increase in various patient conditions, while fast-acting antiplasmins decrease. Oral contraceptive use in women consistently elevates slow antiplasmins.
Area of Science:
- Biochemistry
- Hematology
- Clinical Chemistry
Background:
- Fibrinolysis is a crucial process for blood clot breakdown.
- Antiplasmins regulate fibrinolytic activity.
- Understanding antiplasmin behavior is vital for diagnosing and managing thrombotic and hemorrhagic disorders.
Purpose of the Study:
- To investigate the behavior of slow-acting and fast-acting antiplasmins in patients with various clinical disorders.
- To compare antiplasmin levels in patients with healthy volunteers.
- To assess the impact of oral contraceptives on antiplasmin levels.
Main Methods:
- Fibrinolytic activity was measured by monitoring the decrease in turbidity of heat-treated fibrin powder.
- Residual fibrinolytic activity was determined in serum-plasmin mixtures.
- Antiplasmin behavior was assessed after short and long preincubation times.
Main Results:
- Patients with various clinical disorders showed increased levels of slow-acting antiplasmins.
- Fast-acting antiplasmins were generally decreased in these patients compared to healthy controls.
- Normal women using oral contraceptives exhibited consistently high levels of slow antiplasmins.
- In vitro tests indicated that epsilon-aminocaproic acid, Trasylol, and soybean trypsin inhibitor function exclusively as fast antiplasmins.
Conclusions:
- Alterations in slow-acting and fast-acting antiplasmin levels are associated with diverse clinical conditions.
- Oral contraceptive use significantly influences antiplasmin profiles.
- Specific antifibrinolytic agents act as fast antiplasmins, suggesting targeted therapeutic potential.