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The Multifaceted Benefits of Protein Co-expression in Escherichia coli
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Improving heterologous gene expression in Aspergillus niger by introducing multiple copies of protein-binding
1Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Aims:
To increase the expression level of heterologous gene in Aspergillus niger.
Methods And Results:
Inserting multiple copies of an activator protein-binding site originally encoded in the cis-regulatory region of A. niger glucoamylase gene (glaA), CCAAT-containing sequence, into the promoter of an expression plasmid greatly enhanced the production of heterologous protein.
Conclusion:
The promoter activity was increased by the introduction of the CCAAT-containing sequence resulting in increased gene expression.
Significance And Impact Of The Study:
This study provides evidence that the CCAAT-containing region of the glaA promoter of A. niger is a binding site for a positive transcriptional factor and demonstrates the possibility for improving the expression of both heterologous and endogenous genes in Aspergillus.
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