Application of calorimetric measurements for biokinetic characterisation of nitrifying population in activated sludge

A preliminary investigation is described on the application of calorimetry as a sensitive technique to evaluate nitrifying activity in activated sludge. Calorimetric profiles (thermograms) related to heat dissipation due to biological nitrification reactions (ammonia or nitrite consumption) have been interpreted. Correlations between calorimetric data and the main process variables, i.e. ammonia and nitrite concentration and oxygen uptake, have been verified, and confirm the potential of calorimetry to investigate, monitor and control even weakly exothermic biological processes like autotrophic nitrification. Heat yields (Y(Q/i)) for ammonia, nitrite, and oxygen, defined as the heat released per unit amount of converted reactant, have been separately evaluated. Moreover, calorimetric experiments on activated sludge from a full-scale nitrogen removal wastewater treatment plant have been carried out and kinetic parameters for both ammonia and nitrite oxidising bacteria have been estimated.