A sucrose transporter, LjSUT4, is up-regulated during Lotus japonicus nodule development
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An Induction System for Clustered Stomata by Sugar Solution Immersion Treatment in Arabidopsis thaliana Seedlings
Published on: February 15, 2019
Emmanouil Flemetakis1, Maria Dimou, Daniela Cotzur
1Agricultural University of Athens, Department of Agricultural Biotechnology, Iera Odos 75, 118 55 Athens, Greece.
Abstract:
LjSUT4, encoding a putative sucrose transporter, was identified in a Lotus japonicus nodule cDNA library. The deduced amino acid sequence showed a high degree of identity with sucrose transporters from other plants. Semi-quantitative RT-PCR analysis demonstrated that the L. japonicus SUT4 gene was expressed at high levels in both roots and nodules. In situ hybridization revealed that, in young nodules, SUT4 mRNA transcripts are present in vascular bundles, inner cortex and both infected and uninfected cells while, in mature nodules, accumulation of transcripts was restricted only in vascular bundles and the inner cortex. The results indicated that LjSUT4 codes for a putative sucrose transporter, and its expression pattern suggests a possible shift in the mechanism of sugar transport during nodule development. The role of this polypeptide in sucrose transport and metabolism is discussed.
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Glucose Transporters
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Phloem and Sugar Transport
Transcellular Transport of Solutes
Key Elements for Plant Nutrition
Regulation of Transpiration by Stomata
Water and Mineral Acquisition
